analyses of genetic variation in khorasan indigenous chicken breed by using of microsatellite mark

this study investigated genetic variation within indigenous chicken of khorasan using four microsatellite markers (mcw5, mcw16, mcw18 and mcw34). 90 blood samples were collected from this population and phenol-chloroform extraction method was used to isolate the dna. the highest and the lowest allele number and effective alleles are shown in mcw5 (6 and 5.293) and mcw34 (4 and 3.138), respectively. the expected heterozygosis varied from 0.8157 (mcw5) to 0.6854 (mcw34). the amount of genetic variation within this population was found 0.74 this could be due to low inbreeding in this population. the hardy-weinberg equilibrium (hwe) test showed that all loci deviated from hwe (p<0.005). to short, the results of this study suggested that microsatellite dna markers are useful tools for studying the genetic relationships among native chicken breeds. this is the first genetical study on indigenous chicken of khorasan.